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HspB7 Antibody (OTI1D11) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71833

Novus Biologicals, part of Bio-Techne
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NBP2-71833

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1D11

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human HSPB7(NP_055239) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

18.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HspB7 Antibody (OTI1D11) - Azide and BSA Free

Western Blot: HspB7 Antibody (OTI1D11)Azide and BSA Free [NBP2-71833]

Western Blot: HspB7 Antibody (OTI1D11)Azide and BSA Free [NBP2-71833]

Western Blot: HspB7 Antibody (OTI1D11) - Azide and BSA Free [NBP2-71833] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HspB7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HspB7.
Immunocytochemistry/ Immunofluorescence: HspB7 Antibody (OTI1D11) - Azide and BSA Free [NBP2-71833]

Immunocytochemistry/ Immunofluorescence: HspB7 Antibody (OTI1D11) - Azide and BSA Free [NBP2-71833]

Immunocytochemistry/Immunofluorescence: HspB7 Antibody (OTI1D11) - Azide and BSA Free [NBP2-71833] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HspB7.
CyTOF-ready: HspB7 Antibody (OTI1D11) - Azide and BSA Free [NBP2-71833] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-HspB7 antibody, and then analyzed by flow cytometry.

Applications for HspB7 Antibody (OTI1D11) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HspB7

Alternate Names

Cardiovascular heat shock protein, CVHSP, DKFZp779D0968, FLJ32733, heat shock 27kDa protein family, member 7 (cardiovascular), heat shock protein beta-7, HspB7, member 7 (cardiovascular)

Gene Symbol

HSPB7

Additional HspB7 Products

Product Documents for HspB7 Antibody (OTI1D11) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HspB7 Antibody (OTI1D11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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