Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3C12-H11

Concentration

1 mg/ml

View all HSPB8 Antibodies »

Product Specifications

Immunogen

His-tagged human recombinant HSP22

Localization

Cytoplasm , Nucleus

Specificity

Detects approx 22kDa. Does not cross react with alpha crystallin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for HSPB8/HSP22 Antibody (3C12-H11)

Western Blot: HSPB8/HSP22 Antibody (3C12-H11) [NBP1-48303]

Western Blot: HSPB8/HSP22 Antibody (3C12-H11) [NBP1-48303]

Western Blot: HSPB8/HSP22 Antibody (3C12-H11) [NBP1-48303] - Western Blot analysis of Rat Cell lysates showing detection of Hsp22 protein using Mouse Anti-Hsp22 Monoclonal Antibody, Clone 3C12-H11 (NBP1-48303). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp22 Monoclonal Antibody (NBP1-48303) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunohistochemistry: HSPB8/HSP22 Antibody (3C12-H11) [NBP1-48303]

Immunohistochemistry: HSPB8/HSP22 Antibody (3C12-H11) [NBP1-48303]

Immunohistochemistry: HSPB8/HSP22 Antibody (3C12-H11) [NBP1-48303] - Immunohistochemistry analysis using Mouse Anti-Hsp22 Monoclonal Antibody, Clone 3C12-H11 (NBP1-48303). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp22 Monoclonal Antibody (NBP1-48303) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Granular layer of the epidermis. Some dermal staining.
Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody (3C12-H11) [NBP1-48303]

Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody (3C12-H11) [NBP1-48303]

Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody (3C12-H11) [NBP1-48303] - Mouse backskin sections. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute.

Applications for HSPB8/HSP22 Antibody (3C12-H11)

Application
Recommended Usage

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:2000
Application Notes
1 ug/ml of HSP22 Antibody was sufficient for detection of HSP22 in 20 ug of whole rat tissue extract by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSPB8

Hsp22 (HSPB8) is a 196-amino acid protein that contains a central portion homologous to a highly conserved HSP-alpha crystallin domain common to all the small heat shock protein (HSP20) family members. Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. The highest abundance of Hsp22 is in skeletal muscle, heart, and placenta. Mutations in the HSPB8 gene are associated with the inherited peripheral neuropathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L).

Long Name

Heat Shock Protein beta 8

Alternate Names

CMT2L, CRYAC, DHMN2, E2IG1, H11, HMN2A, HSP22

Gene Symbol

HSPB8

UniProt

Q9UJY2 (Human)

Additional HSPB8 Products

Product Documents for HSPB8/HSP22 Antibody (3C12-H11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSPB8/HSP22 Antibody (3C12-H11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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