HSPB8/HSP22 Antibody (3K10W1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15464

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15464-20ul
NBP3-15464-100ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3K10W1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HSPB8 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HSPB8/HSP22 (Q9UJY1). MADGQMPFSCHYPSRLRRDPFRDSPLSSRLLDDGFGMDPFPDDLTASWPDWALPRLSSAWPGTLRSGMVPRGPTATARFGVPAEGRTPPPFPGEPWKVCV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSPB8/HSP22 Antibody (3K10W1)

Western Blot: HSPB8/HSP22 Antibody (3K10W1) [NBP3-15464]

Western Blot: HSPB8/HSP22 Antibody (3K10W1) [NBP3-15464]

Western Blot: HSPB8/HSP22 Antibody (3K10W1) [NBP3-15464] - Western blot analysis of extracts of various cell lines, using HSPB8/HSP22 Rabbit mAb (NBP3-15464) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: HSPB8/HSP22 Antibody (3K10W1) [NBP3-15464]

Immunocytochemistry/ Immunofluorescence: HSPB8/HSP22 Antibody (3K10W1) [NBP3-15464]

Immunocytochemistry/Immunofluorescence: HSPB8/HSP22 Antibody (3K10W1) [NBP3-15464] - Immunofluorescence analysis of U-2 OS cells using HSPB8/HSP22 Rabbit mAb (NBP3-15464) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: HSPB8/HSP22 Antibody (3K10W1) [NBP3-15464]

Immunocytochemistry/ Immunofluorescence: HSPB8/HSP22 Antibody (3K10W1) [NBP3-15464]

Immunocytochemistry/Immunofluorescence: HSPB8/HSP22 Antibody (3K10W1) [NBP3-15464] - Immunofluorescence analysis of NIH-3T3 cells using HSPB8/HSP22 Rabbit mAb (NBP3-15464) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for HSPB8/HSP22 Antibody (3K10W1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSPB8

HSP22 is encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.

Long Name

Heat Shock Protein beta 8

Alternate Names

CMT2L, CRYAC, DHMN2, E2IG1, H11, HMN2A, HSP22

Gene Symbol

HSPB8

Additional HSPB8 Products

Product Documents for HSPB8/HSP22 Antibody (3K10W1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSPB8/HSP22 Antibody (3K10W1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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