HSPB8/HSP22 Antibody (5C3)

Novus Biologicals, part of Bio-Techne | Catalog # H00026353-M03

Novus Biologicals, part of Bio-Techne
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H00026353-M03
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 5C3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HSPB8 Antibodies »

Product Specifications

Immunogen

HSPB8 (NP_055180, 97 a.a. ~ 196 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KVCVNVHSFKPEELMVKTKDGYVEVSGKHEEKQQEGGIVSKNFTKKIQLPAEVDPVTVFASLSPEGLLIIEAPQVPPYSTFGESSFNNELPQDSQEVTCT

Reactivity Notes

Human. Other species not tested.

Specificity

HSPB8 (5C3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for HSPB8/HSP22 Antibody (5C3)

Western Blot: HSPB8/HSP22 Antibody (5C3) [H00026353-M03]

Western Blot: HSPB8/HSP22 Antibody (5C3) [H00026353-M03]

Western Blot: HSPB8/HSP22 Antibody (5C3) [H00026353-M03] - Analysis of HSPB8 expression in transfected 293T cell line by HSPB8 monoclonal antibody (M03), clone 5C3. Lane 1: HSPB8 transfected lysatE (35.446 KDa). Lane 2: Non-transfected lysate.
ELISA: HSPB8/HSP22 Antibody (5C3) [H00026353-M03]

ELISA: HSPB8/HSP22 Antibody (5C3) [H00026353-M03]

ELISA: HSPB8/HSP22 Antibody (5C3) [H00026353-M03] - Detection limit for recombinant GST tagged HSPB8 is approximately 1ng/ml as a capture antibody.

Applications for HSPB8/HSP22 Antibody (5C3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HSPB8

The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.

Long Name

Heat Shock Protein beta 8

Alternate Names

CMT2L, CRYAC, DHMN2, E2IG1, H11, HMN2A, HSP22

Entrez Gene IDs

26353 (Human)

Gene Symbol

HSPB8

OMIM

158590 (Human)

UniProt

NP_055180 (Human)

Additional HSPB8 Products

Product Documents for HSPB8/HSP22 Antibody (5C3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HSPB8/HSP22 Antibody (5C3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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