HSPB8/HSP22 Antibody (OTI1E3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02576
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI1E3
Concentration
0.88 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human HSPB8(NP_055180) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
21.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for HSPB8/HSP22 Antibody (OTI1E3)
Western Blot: Hsp22 Antibody (1E3) [NBP2-02576] Analysis of extracts (35ug) from 9 different cell lines by using anti-Hsp22 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: HSPB8/HSP22 Antibody (OTI1E3) [NBP2-02576]
Immunocytochemistry/Immunofluorescence: Hsp22 Antibody (1E3) [NBP2-02576] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hsp22.Flow Cytometry: HSPB8/HSP22 Antibody (OTI1E3) [NBP2-02576]
Flow Cytometry: Hsp22 Antibody (1E3) [NBP2-02576] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Hsp22 antibody, and then analyzed by flow cytometry.Applications for HSPB8/HSP22 Antibody (OTI1E3)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:500-2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.88 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HSPB8
Long Name
Heat Shock Protein beta 8
Alternate Names
CMT2L, CRYAC, DHMN2, E2IG1, H11, HMN2A, HSP22
Entrez Gene IDs
26353 (Human)
Gene Symbol
HSPB8
Additional HSPB8 Products
Product Documents for HSPB8/HSP22 Antibody (OTI1E3)
Product Specific Notices for HSPB8/HSP22 Antibody (OTI1E3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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