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HSPB8/HSP22 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1123

Novus Biologicals, part of Bio-Techne
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NB100-1123

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence NELPQDSQEVTCT corresponding to C-Terminus according to NP_055180.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for HSPB8/HSP22 Antibody

Western Blot: HSPB8/HSP22 Antibody [NB100-1123]

Western Blot: HSPB8/HSP22 Antibody [NB100-1123]

Western Blot: HSPB8/HSP22 Antibody [NB100-1123] - Staining of Human Muscle lysate (RIPA buffer, 30 ug total protein per lane). Antibody at 1 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody [NB100-1123]

Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody [NB100-1123]

Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody [NB100-1123] - Staining of paraffin embedded Human Tonsil. Antibody at 10 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: HSPB8/HSP22 Antibody [NB100-1123]

Western Blot: HSPB8/HSP22 Antibody [NB100-1123]

Western Blot: HSPB8/HSP22 Antibody [NB100-1123] - Analysis of H11/HSP22 in Human Muscle lysate using NB100-1123 at 1 ug/mL.

Applications for HSPB8/HSP22 Antibody

Application
Recommended Usage

Immunohistochemistry

10 ug/mL

Immunohistochemistry-Paraffin

10 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.5 - 1 ug/mL
Application Notes
WB: Approx. 20-25 kDa band observed in human skeletal muscle lysate (predicted MW of ~22 kDa band according to NP_055180).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSPB8

Hsp22 (HSPB8) is a 196-amino acid protein that contains a central portion homologous to a highly conserved HSP-alpha crystallin domain common to all the small heat shock protein (HSP20) family members. Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. The highest abundance of Hsp22 is in skeletal muscle, heart, and placenta. Mutations in the HSPB8 gene are associated with the inherited peripheral neuropathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L).

Long Name

Heat Shock Protein beta 8

Alternate Names

CMT2L, CRYAC, DHMN2, E2IG1, H11, HMN2A, HSP22

Entrez Gene IDs

26353 (Human); 62353 (Mouse)

Gene Symbol

HSPB8

Additional HSPB8 Products

Product Documents for HSPB8/HSP22 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HSPB8/HSP22 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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