HSPB8/HSP22 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84945

Novus Biologicals, part of Bio-Techne
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NBP1-84945
NBP1-84945-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HSPB8 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LLDDGFGMDPFPDDLTASWPDWALPRLSSAWPGTLRSGMVPRGPTATARFGVPAEGRTPPPFPGEPWKVCVNVHSFKPEELMVKTKDGYVEVSGKHEEKQQEGGIVSKNFTKKIQLP

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSPB8/HSP22 Antibody

Western Blot: HSPB8/HSP22 Antibody [NBP1-84945]

Western Blot: HSPB8/HSP22 Antibody [NBP1-84945]

Western Blot: HSPB8/HSP22 Antibody [NBP1-84945] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HSPB8 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: HSPB8/HSP22 Antibody [NBP1-84945]

Immunocytochemistry/ Immunofluorescence: HSPB8/HSP22 Antibody [NBP1-84945]

Immunocytochemistry/Immunofluorescence: HSPB8/HSP22 Antibody [NBP1-84945] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody [NBP1-84945]

Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody [NBP1-84945]

Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody [NBP1-84945] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
View 3 more images

Applications for HSPB8/HSP22 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSPB8

HSP22 is encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.

Long Name

Heat Shock Protein beta 8

Alternate Names

CMT2L, CRYAC, DHMN2, E2IG1, H11, HMN2A, HSP22

Gene Symbol

HSPB8

Additional HSPB8 Products

Product Documents for HSPB8/HSP22 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSPB8/HSP22 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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