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HSPH1/HSP105 Antibody (J1G12) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-04304

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # J1G12

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Recombinant human HSPH1/HSP105 (1-858 aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for HSPH1/HSP105 Antibody (J1G12) - BSA Free

Western Blot: HSPH1/HSP105 Antibody (J1G12)BSA Free [NBP1-04304]

Western Blot: HSPH1/HSP105 Antibody (J1G12)BSA Free [NBP1-04304]

Western Blot: HSPH1/HSP105 Antibody (J1G12) [NBP1-04304] - Cell lysates of HeLa(30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hsp105 alpha (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Applications for HSPH1/HSP105 Antibody (J1G12) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSPH1

Hsp105 is a mammalian member of the HSP105/110 family, a diverged subgroup of the HSP70 family. HSP105 exists as two isoforms, alpha and beta. Hsp105 alpha associates with Hsp70/Hsc70 as complexes in vivo and regulates the chaperone activity of Hsp70/Hsc70 negatively in vitro and in vivo. Hsp105 alpha is constitutively expressed and also induced by various stress, whereas Hsp105 beta, lacking 44 amino acids from Hsp105 alpha, is specifically expressed during mild heat shock. Although Hsp105 alpha is shown to localize in the cytoplasm of mammalian cells, cellular localization of Hsp105 beta is not known

Long Name

Heat Shock Protein H1

Alternate Names

HSP105a, HSP110, NY-CO-25

Gene Symbol

HSPH1

Additional HSPH1 Products

Product Documents for HSPH1/HSP105 Antibody (J1G12) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HSPH1/HSP105 Antibody (J1G12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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