HuC Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-02943

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HuC Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 161-260 of human HuC (NP_001411.2). KTGQALLTHLYQSSAR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HuC Antibody - Azide and BSA Free

Western Blot: HuC AntibodyAzide and BSA Free [NBP3-02943]

Western Blot: HuC AntibodyAzide and BSA Free [NBP3-02943]

Western Blot: HuC Antibody [NBP3-02943] - Analysis of extracts of various cell lines, using HuC antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit
Immunocytochemistry/ Immunofluorescence: HuC Antibody - Azide and BSA Free [NBP3-02943]

Immunocytochemistry/ Immunofluorescence: HuC Antibody - Azide and BSA Free [NBP3-02943]

Immunocytochemistry/Immunofluorescence: HuC Antibody [NBP3-02943] - Analysis of SH-SY5Y cells using ELAVL3 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. HuC antibody
Immunohistochemistry-Paraffin: HuC Antibody - Azide and BSA Free [NBP3-02943]

Immunohistochemistry-Paraffin: HuC Antibody - Azide and BSA Free [NBP3-02943]

Immunohistochemistry-Paraffin: HuC Antibody [NBP3-02943] - Immunohistochemistry of paraffin-embedded mouse brain using HuC antibody (NBP3-02943) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for HuC Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HuC

HuC is a member of the ELAVL protein family, ELAV-like 3 is a neural-specific RNA-binding protein which contains three RNP-type RNA recognition motifs. The observation that ELAVL3 is one of several Hu antigens (neuronal-specific RNA-binding proteins) recognized by the anti-Hu serum antibody present in sera from patients with paraneoplastic encephalomyelitis and sensory neuronopathy (PEM/PSN) suggests it has a role in neurogenesis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

DKFZp547J036, ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C), ELAV-like protein 3, Hu antigen C, Hu-antigen C, HuC, HUCL, HUCParaneoplastic cerebellar degeneration-associated antigen, MGC20653, PLE21Paraneoplastic limbic encephalitis antigen 21

Gene Symbol

ELAVL3

Additional HuC Products

Product Documents for HuC Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HuC Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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