HuC Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13367

Novus Biologicals, part of Bio-Techne
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NBP3-13367
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Human (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HuC Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse HuC. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for HuC Antibody

Western Blot: HuC Antibody [NBP3-13367]

Western Blot: HuC Antibody [NBP3-13367]

Western Blot: HuC Antibody [NBP3-13367] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HuC antibody (NBP3-13367) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: HuC Antibody [NBP3-13367]

Immunocytochemistry/ Immunofluorescence: HuC Antibody [NBP3-13367]

Immunocytochemistry/Immunofluorescence: HuC Antibody [NBP3-13367] - HuC antibody detects HuC protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HuC stained by HuC antibody (NBP3-13367) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:1000. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Frozen: HuC Antibody [NBP3-13367]

Immunohistochemistry-Frozen: HuC Antibody [NBP3-13367]

Immunohistochemistry-Frozen: HuC Antibody [NBP3-13367] - HuC antibody detects HuC protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse hippocampus. Green: HuC stained by HuC antibody (NBP3-13367) diluted at 1:250. Blue: Fluoroshield with DAPI.
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Applications for HuC Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HuC

HuC is a member of the ELAVL protein family, ELAV-like 3 is a neural-specific RNA-binding protein which contains three RNP-type RNA recognition motifs. The observation that ELAVL3 is one of several Hu antigens (neuronal-specific RNA-binding proteins) recognized by the anti-Hu serum antibody present in sera from patients with paraneoplastic encephalomyelitis and sensory neuronopathy (PEM/PSN) suggests it has a role in neurogenesis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

DKFZp547J036, ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C), ELAV-like protein 3, Hu antigen C, Hu-antigen C, HuC, HUCL, HUCParaneoplastic cerebellar degeneration-associated antigen, MGC20653, PLE21Paraneoplastic limbic encephalitis antigen 21

Gene Symbol

ELAVL3

Additional HuC Products

Product Documents for HuC Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HuC Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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