Human 17 beta-HSD14/HSD17B14 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7515
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 736043
Product Specifications
Immunogen
E. coli-derived recombinant human 17 beta‑HSD14/HSD17B14
Ala2-Ser270
Accession # Q9BPX1
Ala2-Ser270
Accession # Q9BPX1
Specificity
Detects human 17 beta‑HSD14/HSD17B14 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human 17 beta-HSD14/HSD17B14 Antibody
17 beta‑HSD14/HSD17B14 in Human Brain.
17 beta-HSD14/HSD17B14 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human 17 beta-HSD14/HSD17B14 Monoclonal Antibody (Catalog # MAB7515) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human 17 beta-HSD14/HSD17B14 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (medulla)
Sample: Immersion fixed paraffin-embedded sections of human brain (medulla)
Reviewed Applications
Read 2 reviews rated 5 using MAB7515 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 17 beta-HSD14/HSD17B14
Long Name
17 beta Hydroxysteroid Dehydrogenase 14
Alternate Names
17 betaHSD14, DHRS10, HSD17B14, retSDR3, SDR3, SDR47C1
Gene Symbol
HSD17B14
UniProt
Additional 17 beta-HSD14/HSD17B14 Products
Product Documents for Human 17 beta-HSD14/HSD17B14 Antibody
Product Specific Notices for Human 17 beta-HSD14/HSD17B14 Antibody
For research use only
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