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Human ADAMTS10 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5270

R&D Systems, part of Bio-Techne
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MAB5270
MAB5270-SP

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 496708

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS10
Phe26-Gly1102
Accession # Q9H324

Specificity

Detects human ADAMTS10 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human ADAMTS1, 3, 4, 5, 12, 13, 15, 16, or L2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human ADAMTS10 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAMTS10, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human ADAMTS10 under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAMTS10

ADAMTS10 belongs to the ADAMTS (a disintegrin and metalloproteinase domain with thrombospondin type-1 motifs) family of zinc-dependent metalloproteases. They have been demonstrated to have important roles in connective tissue organization, coagulation, inflammation, arthritis, angiogenesis and cell migration. Mutations in the ADAMTS10 gene may cause autosomal recessive Weill-Marchesani syndrome. The amino acid sequence of human ADAMTS10 is 100%, 96% and 94% identical to that of chimpanzee, dog and mouse.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 10

Alternate Names

a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 10, ADAM metallopeptidase with thrombospondin type 1 motif, 10, ADAM-TS 10, ADAMTS-10, ADAM-TS10A disintegrin and metalloproteinase with thrombospondin motifs 10, EC 3.4.24.-, EC 3.4.24.82, WMS, zinc metalloendopeptidase

Entrez Gene IDs

81794 (Human); 224697 (Mouse); 362847 (Rat)

Gene Symbol

ADAMTS10

UniProt

Additional ADAMTS10 Products

Product Documents for Human ADAMTS10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human ADAMTS10 Antibody

For research use only

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