Human Adrenomedullin/ADM Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6108

R&D Systems, part of Bio-Techne
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MAB6108
MAB6108-SP
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 357923

View all Adrenomedullin/ADM Antibodies »

Product Summary for Human Adrenomedullin/ADM Antibody

Immunogen

E. coli-derived recombinant human Adrenomedullin/ADM
Ala22-Gly147
Accession # P35318

Specificity

Detects human Adrenomedullin/ADM in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Adrenomedullin/ADM Antibody

Adrenomedullin/ADM antibody in Human Heart by Immunohistochemistry (IHC-P).

Adrenomedullin/ADM in Human Heart.

Adrenomedullin/ADM was detected in immersion fixed paraffin-embedded sections of human heart using Human Adrenomedullin/ADM Monoclonal Antibody (Catalog # MAB6108) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasma in cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Adrenomedullin/ADM Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB6108 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adrenomedullin/ADM

Adrenomedullin (ADM) is a secreted, monomeric, 6 kDa member of the adrenomedullin family of molecules.  It is widely expressed, being found in smooth muscle cells, endothelium, adrenal medulla chromaffin cells, fibroblasts and keratinocytes.  Adrenomedullin has multiple functions including vasodilation, the maintenance of vascular integrity, and the suppression of inflammatory mediator secretion.  The human Adrenomedullin preproprecursor is 185 amino acids (aa) in length.  It contains a 21 aa signal sequence, a processed 20 aa peptide termed PAMP (aa 22-41), an N-terminal propeptide (aa 45-92), the Adrenomedullin precursor (amidation is required for maturation) (aa 95-147), and a C-terminal propeptide (aa 148-185).  The Adrenomedullin precursor with a terminal Gly147 circulates naturally with bioactive, mature amidated Adrenomedullin (aa 95-146).  Depending upon the tissue, truncated forms of Adrenomedullin likely also occur, including variants spanning aa 120-146 and 128-146.  Over aa 22-147, the human Adrenomedullin proprecursor shares 70% aa identity with mouse Adrenomedullin proprecursor.       

Alternate Names

ADM

Entrez Gene IDs

133 (Human); 11535 (Mouse); 25026 (Rat)

Gene Symbol

ADM

UniProt

P35318 (Human)

Additional Adrenomedullin/ADM Products

Product Documents for Human Adrenomedullin/ADM Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Adrenomedullin/ADM Antibody

For research use only

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