Human AICDA Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB39102

R&D Systems, part of Bio-Techne
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MAB39102
MAB39102-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 359218

View all AICDA Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human AICDA
Met1-Leu198
Accession # Q9GZX7

Specificity

Detects human AICDA in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human AICDA Antibody

AICDA antibody in Ramos Human Cell Line by Immunocytochemistry (ICC).

AICDA in Ramos Human Cell Line.

AICDA was detected in immersion fixed Ramos human Burkitt's lymphoma cell line using Rat Anti-Human AICDA Monoclonal Antibody (Catalog # MAB39102) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human AICDA Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Ramos human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AICDA

AICDA, also known as AID, is a 24 kDa cytidine deaminase that converts deoxycytidine to deoxyuridine in single stranded DNA. Its expression is restricted to activated lymph node and tonsillar B cells and is required for terminal B cell differentiation. Loss of function mutations of AICDA cause severe humoral immunodeficiency by preventing immunoglobulin class switching and somatic hypermutation. Human AICDA shares 92% - 95% amino acid sequence identity with bovine, canine, and mouse AICDA.

Long Name

Activation-Induced Cytidine Deaminase

Alternate Names

AID, Arp2, CDA2, HIGM2

Entrez Gene IDs

57379 (Human); 11628 (Mouse); 399679 (Rat)

Gene Symbol

AICDA

UniProt

Q9GZX7

Additional AICDA Products

Product Documents for Human AICDA Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human AICDA Antibody

For research use only

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