Human Ajuba Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5594
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Ajuba
Met1-Arg121
Accession # Q96IF1
Met1-Arg121
Accession # Q96IF1
Specificity
Detects endogenous human Ajuba in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Ajuba Antibody
Detection of Human Ajuba by Western Blot.
Western blot shows lysates of PC-3 human prostate cancer cell line, A549 human lung carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Human Ajuba Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5594) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Ajuba at approximately 55 to 60 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Human Ajuba Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: PC-3 human prostate cancer cell line, A549 human lung carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line
Sample: PC-3 human prostate cancer cell line, A549 human lung carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ajuba
Ajuba (an Urdu word meaning "curiosity") is a 55‑60 kDa member of the Zyxin/Ajuba family of proteins. Ajuba is found in keratinocytes, astrocytes and neurons, and exhibits multiple functions. It shuttles between the nucleus and cytosol, and participates in alpha-catenin:F-actin binding, beta-catenin binding and phosphorylation, p62 aPKC scaffold protein binding and NF-kappa B activation, and Aurora-A binding and phosphorylation. Human Ajuba is 538 amino acids (aa) in length. It contains a Pro-rich preLIM region that binds actin and Grb2 (aa 1‑335), an NES (aa 280‑287), and three LIM domains that bind multiple proteins (aa 336‑397, 401‑461 and 462‑530).
Alternate Names
JUB
Gene Symbol
AJUBA
UniProt
Additional Ajuba Products
Product Documents for Human Ajuba Antibody
Product Specific Notices for Human Ajuba Antibody
For research use only
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