Human alpha-Defensin 1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8198
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Immunohistochemistry
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human alpha‑Defensin 1
Ala65-Cys94
Accession # P59665
Ala65-Cys94
Accession # P59665
Specificity
Detects human alpha‑Defensin 1 in direct ELISAs.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human alpha-Defensin 1 Antibody
alpha‑Defensin 1 in Human Small Intestine.
a-Defensin 1 was detected in formalin fixed paraffin-embedded sections of human small intestine using Sheep Anti-Human a-Defensin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8198) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to lamina propria in intestinal villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Human alpha-Defensin 1 ELISA Standard Curve.
Recombinant Human a-Defensin 1 protein was serially diluted 2-fold and captured by Mouse Anti-Human a-Defensin 1 Monoclonal Antibody (Catalog # MAB8198) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human a-Defensin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8198) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human alpha-Defensin 1 Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human alpha‑Defensin 1 Monoclonal Antibody (Catalog # MAB8198).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human alpha-Defensin 1 DuoSet ELISA Kit (Catalog # DY8198-05) for convenient development of a sandwich ELISA.
Immunohistochemistry
5-15 µg/mL
Sample: Formalin fixed paraffin-embedded sections of human small intestine
Sample: Formalin fixed paraffin-embedded sections of human small intestine
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-Defensin 1
Human Defensin alpha 1 (DEFA1) is 94 amino acids (aa) in length and is cleaved into the following chains: HP 1-56 (aa 39-94), Neutrophil defensin 1 (aa 65-94) and Neutrophil defensin 2 ( aa 66-94). Defensins are secreted peptides that kill microbes by permeabilizing their plasma membrane. DEFA1 has antimicrobial activity against Gram-negative and Gram-positive bacteria. ADP-ribosylation reduces cytotoxic and antibacterial activities, it is also known to enhance IL8 production. Phosphorylation at Tyr-85 is reported in several cancer cell lines and interferes with ADP-ribosylation of DEFA1.
Alternate Names
alphaDefensin 1, DEF1, DEFA1, HNP-1, HP1, MRS
Gene Symbol
DEFA1
UniProt
Additional alpha-Defensin 1 Products
Product Documents for Human alpha-Defensin 1 Antibody
Product Specific Notices for Human alpha-Defensin 1 Antibody
For research use only
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