Human alpha-Defensin 5 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10248

R&D Systems, part of Bio-Techne
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MAB10248-100
MAB10248-SP
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Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 972207

View all alpha-Defensin 5 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human alpha-Defensin 5
Thr64-Arg94
Accession # Q01523

Specificity

Detects human alpha-Defensin 5 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human alpha-Defensin 5 Antibody

Human a-Defensin 5 Antibody in ELISA Standard Curve.

Human alpha-Defensin 5 ELISA Standard Curve.

Recombinant Human a-Defensin 5 protein was serially diluted 2-fold and captured by Mouse Anti-Human a-Defensin 5 Monoclonal Antibody (Catalog # MAB10248) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human a-Defensin 5 Monoclonal Antibody (Catalog # MAB102481) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human alpha-Defensin 5 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human alpha-Defensin 5 Monoclonal Antibody (Catalog # MAB102481).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Defensin 5

Defensin alpha 5 (DEFA5), also known as human alpha defensin 5 (HD5), is a secreted antimicrobial protein encoded by the DEFA5 gene and a member of the Defensins family. Defensins are thought to kill microbes by permeabilizing their plasma membrane. DEFA5 is expressed in Paneth cells, which are secretory epithelial cells in the small intestine. Unlike other defensins, DEFA5 is stored in secretory vesicles as a propeptide, and is processed into different peptides with different potency and localization.

Long Name

Defensin Alpha 5

Alternate Names

DEF5, DEFA5, Defensin alpha 5, HD-5

Entrez Gene IDs

1670 (Human)

Gene Symbol

DEFA5

UniProt

Q01523

Additional alpha-Defensin 5 Products

Product Documents for Human alpha-Defensin 5 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human alpha-Defensin 5 Antibody

For research use only

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