Human alpha-Internexin Antibody

Name: Human alpha-Internexin Antibody
Brand: R&D Systems
SKU: MAB4844

R&D Systems, part of Bio-Techne | Catalog # MAB4844

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 529724

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Product Specifications

Immunogen

E. coli-derived recombinant human alpha-Internexin
Val230-Glu450
Accession # Q16352

Specificity

Detects human alpha‑Internexin in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) NF-M or rhVimentin is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human alpha-Internexin Antibody

Detection of Human alpha-Internexin by Western Blot.

Western blot shows lysates of human cortex tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human a-Internexin Monoclonal Antibody (Catalog # MAB4844) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for a-Internexin at approximately 50-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human alpha-Internexin Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Western Blot

2 µg/mL
Sample: Human cortex tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Internexin

alpha-Internexin ( alpha-Inx; also NF-66) is a 66 kDa member of the intermediate filament (IF) protein family. It was the first of two molecules named for its presumed interaction with cytoskeletal proteins. alpha-Inx is one of four Class IV neuronal IF proteins. It both self-assembles and complexes with NF-L, H and M in select cortical and cerebellar neurons. alpha-Inx contains one DNA-binding region (aa 10‑92), a poly‑Glu segment (aa 449‑454) and three serine phosphorylation sites (Ser72/335/496). There is one 494 aa alternative splice form that shows multiple short sequence aa substitutions in the first 200 amino acids. Over aa 230‑450, human alpha-Inx shows more that 96% aa identity with mouse, canine and rat alpha-Inx.

Alternate Names

alphaInternexin, INA, NEF5, NF-66, TXBP-1

Entrez Gene IDs

9118 (Human); 226180 (Mouse); 24503 (Rat)

Gene Symbol

INA

UniProt

Q16352 (Human)

Additional alpha-Internexin Products

Product Documents for Human alpha-Internexin Antibody

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COA

SDS

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Product Specific Notices for Human alpha-Internexin Antibody

For research use only

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