Human AMIGO Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2565
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human AMIGO
Met1-Gly365
Accession # Q86WK6
Met1-Gly365
Accession # Q86WK6
Specificity
Detects human AMIGO in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) AMIGO2 is observed and less than 1% cross-reactivity with rhAMIGO3 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human AMIGO Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human AMIGO
Sample: Recombinant Human AMIGO
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: AMIGO
AMIGO is a 65 kDa type I transmembrane glycoprotein that belongs to both the Ig- and LLR (leucine-rich repeat)- superfamilies. It is expressed on developing neuronal fiber tracts and participates in their formation. AMIGO forms both homophilic and heterophilic complexes with AMIGO2 and 3, suggesting an adhesive function for the molecule. Human AMIGO extracellular domain is 87% amino acid identical to mouse AMIGO, and 40% identical to human AMIGO2 and 3.
Long Name
Amphoterin-induced Gene and ORF
Alternate Names
ali2, Alivin-2, AMIGO1
Gene Symbol
AMIGO1
UniProt
Additional AMIGO Products
Product Documents for Human AMIGO Antibody
Product Specific Notices for Human AMIGO Antibody
For research use only
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