Human Aminoacylase/ACY1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2900
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
ELISA Development (Capture)
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 475626
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Aminoacylase/ACY1
Thr2-Ser408
Accession # Q03154
Thr2-Ser408
Accession # Q03154
Specificity
Detects recombinant human Aminoacylase/ACY1 in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse ACY1 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Applications for Human Aminoacylase/ACY1 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Aminoacylase/ACY1 (Catalog # 2900-ZN), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Aminoacylase/ACY1 (Catalog # 2900-ZN), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Aminoacylase/ACY1 (Catalog # 2900-ZN)
Sample: Recombinant Human Aminoacylase/ACY1 (Catalog # 2900-ZN)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aminoacylase/ACY1
The human ACY1 gene encodes Aminoacylase, a member of the M20 family of metalloproteases (1). ACY1 plays a general role in the cytosolic breakdown of acetylated amino acids generated during protein degradation. It also interacts with sphingosine kinase type 1, which is involved in promoting cell growth and inhibiting apoptosis of tumor cells (2). The full-length protein is expressed and the purified enzyme is active as described in Activity Assay Protocol.
References
- Lindner, H.A. et al. (2003) J. Biol. Chem. 278:44496.
- Maceyka, M. et al. (2004) FEBS Lett. 568:30.
Alternate Names
ACY1
Gene Symbol
ACY1
UniProt
Additional Aminoacylase/ACY1 Products
Product Documents for Human Aminoacylase/ACY1 Antibody
Product Specific Notices for Human Aminoacylase/ACY1 Antibody
For research use only
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