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Human Aminopeptidase O/ONPEP Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4647

R&D Systems, part of Bio-Techne
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MAB4647
MAB4647-SP

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 562912

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Aminopeptidase O/ONPEP
Met1-Phe819
Accession # Q8N6M6

Specificity

Detects human Aminopeptidase O/ONPEP in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Aminopeptidase O/ONPEP Antibody

Aminopeptidase O/ONPEP antibody in Human Kidney by Immunohistochemistry (IHC-P).

Aminopeptidase O/ONPEP in Human Kidney.

Aminopeptidase O/ONPEP was detected in immersion fixed paraffin-embedded sections of human kidney array using Human Aminopeptidase O/ONPEP Monoclonal Antibody (Catalog # MAB4647) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Aminopeptidase O/ONPEP Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney and human kidney cancer tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aminopeptidase O/ONPEP

Aminopeptidase O (AP-O; also C9orf3) is a 93 kDa (predicted), intracellular member of the M1 metalloprotease family of enzymes. It is expressed in heart, testis and pancreas where it apparently cleaves N terminal Arg and Asn residues. Human Aminopeptidase O is 819 amino acids in length. It contains one catalytic domain (aa 167‑589) with a Zn-binding site (His479-His483) and a C terminal ARM region (aa 706-819). At least three additional isoforms exist. One shows a deletion of aa 455‑553; a second has an 11 aa substitution for the C terminal 265 amino acids; and a third shows both a deletion of aa 455‑553 and an 11 aa substitution for the C-terminal 46 amino acids. Full length human Aminopeptidase O is 76% aa identical to mouse Aminopeptidase O.

Alternate Names

APO, C90RF3, C9orf3, ONPEP

Entrez Gene IDs

84909 (Human); 72061 (Mouse); 290963 (Rat)

Gene Symbol

AOPEP

UniProt

Additional Aminopeptidase O/ONPEP Products

Product Documents for Human Aminopeptidase O/ONPEP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Aminopeptidase O/ONPEP Antibody

For research use only

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