Human Aminopeptidase P1/XPNPEP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2970
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 409111
Product Specifications
Immunogen
E. coli-derived recombinant human Aminopeptidase P1/XPNPEP1
Met1-Gln622
Accession # Q9NQW7
Met1-Gln622
Accession # Q9NQW7
Specificity
Detects human Aminopeptidase P1/XPNPEP1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) XPNPEP1, rmXPNPEP2, or recombinant human XPNPEP2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human Aminopeptidase P1/XPNPEP1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human Aminopeptidase P1/XPNPEP1 (Catalog # 2970-ZN)
Sample: Recombinant Human Aminopeptidase P1/XPNPEP1 (Catalog # 2970-ZN)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aminopeptidase P1/XPNPEP1
The human XPNPEP1 gene encodes aminopeptidase P1 (APP1), which is also known as X-prolyl aminopeptidase with gene aliases of SAMP, XPNPEP, XPNPEPL, and XPNPEPL1 (1‑3). It is a member of the M24 family of metalloproteases, which also contains methionine aminopeptidases, X-Pro dipeptidase, aminopeptidase P2, aminopeptidase P homolog, proliferation-associated protein 1, and suppressor of Ty homolog or chromatin-specific transcription elongation factor large subunit (4). It is a soluble enzyme, in contrast to the GPI-anchored APP2 encoded by XPNPEP2 (5). Human APP1 is widely expressed (3). APP1 removes a N-terminal amino acid from a peptide that contains a Pro residue at the second position. The amino acid sequence of human APP1 is 99%, 97%, 95%, 74%, and 73% identical to that of canine, bovine, mouse/rat, Xenopus, and zebrafish, respectively.
References
- Cottrell, G.S. et al. (2000) Biochemistry 39:15121.
- Sprinkle, T.J. et al. (2000) Arch. Biochem. Biophys. 378:51.
- Vanhoof, G. et al. (1997) Cytogenet. Cell Genet. 78:275.
- Turner, A.J. and G.S. Cottrell (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 931, Elsevier Academic Press, San Diego.
- Simmons, W.H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 934, Elsevier Academic Press, San Diego.
Alternate Names
APP1, SAMP, XPNPEP1
Entrez Gene IDs
7511 (Human)
Gene Symbol
XPNPEP1
UniProt
Additional Aminopeptidase P1/XPNPEP1 Products
Product Documents for Human Aminopeptidase P1/XPNPEP1 Antibody
Product Specific Notices for Human Aminopeptidase P1/XPNPEP1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...