Human Angiopoietin-like Protein 3/ANGPTL3 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3829

R&D Systems, part of Bio-Techne
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AF3829
AF3829-SP
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Conjugate
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Biotin
BAF3829
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Angiopoietin-like Protein 3/ANGPTL3 Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Angiopoietin-like 3 (R&D Systems, Catalog # 3829-AN)
Ser17-Glu460
Accession # Q9Y5C1

Specificity

Detects human Angiopoietin-like 3 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse Angiopoietin-like 3 is observed and less than 1% cross-reactivity with recombinant human (rh) Angiopoietin-like 1 andrhAngiopoietin-like 4 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human Angiopoietin-like Protein 3/ANGPTL3 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Angiopoietin-like 3 (Catalog # 3829-AN)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 3/ANGPTL3

Angiopoietin-like 3 (ANGPTL3), also known as angiopoietin-5, is a secreted, hepatic angiopoietin-like glycoprotein that is structurally related to angiopoietins. Human ANGPTL3 contains an N‑terminal heparin-binding motif, a coiled-coil domain (CCD) that mediates the formation of non-covalent higher-order oligomers, and a C‑terminal fibrinogen-like domain that binds integrin  alphav beta3. In vivo, ANGPTL3 is proteolytically cleaved between the CCD and the fibrinogen-like domain, generating an N-terminal fragment that retains the inhibitory activity on lipoprotein lipase and a C‑terminal fragment that can induce angiogenesis. Full length and cleaved ANGPTL3 circulate in plasma. Mature human ANGPTL3 shares 76% and 83% aa sequence identity with mouse and porcine ANGPTL3, respectively.

Alternate Names

AGNPT5, Ang-5, Angiopoietin-5, ANGPTL3

Entrez Gene IDs

27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)

Gene Symbol

ANGPTL3

UniProt

Q9Y5C1 (Human)

Additional Angiopoietin-like Protein 3/ANGPTL3 Products

Product Documents for Human Angiopoietin-like Protein 3/ANGPTL3 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Angiopoietin-like Protein 3/ANGPTL3 Antibody

For research use only

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