Human AP-2 epsilon Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5060
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human AP-2 epsilon
Ala123-Val217
Accession # Q6VUC0
Ala123-Val217
Accession # Q6VUC0
Specificity
Detects human AP-2 epsilon in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human (rh) AP‑2 alpha, rhAP‑2 beta, rhAP‑2 delta, and rhAP‑2 gamma is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human AP-2 epsilon Antibody
Detection of Human AP-2 epsilon by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human AP-2e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5060) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for AP-2e at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human AP-2 epsilon Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: A431 human epithelial carcinoma cell line and human placenta tissue
Sample: A431 human epithelial carcinoma cell line and human placenta tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: AP-2 epsilon
AP-2 epsilon (Activating protein 2 epsilon) is a 48 kDa member of the AP-2 transcription factor family. It is found in embryonic olfactory bulb mitral cells and adult keratinocytes and chondrocytes. In the nucleus, it presumably forms homodimers and heterodimers with other AP‑2 family members. Human AP‑2 epsilon is 442 amino acids in length. It contains a Gln/Pro-rich transactivation domain (aa 22‑122), a DNA binding domain (aa 216‑286) and an AP‑2, helix‑span‑helix dimerization region (aa 287‑417). There is one potential alternate start site at Met09. Over aa 123‑217, human AP‑2 epsilon shares 86% aa identity with mouse AP‑2 epsilon.
Long Name
Activating Enhancer-binding Protein epsilon
Alternate Names
AP2 epsilon, TFAP2E
Entrez Gene IDs
339488 (Human)
Gene Symbol
TFAP2E
UniProt
Additional AP-2 epsilon Products
Product Documents for Human AP-2 epsilon Antibody
Product Specific Notices for Human AP-2 epsilon Antibody
For research use only
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