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Human Apolipoprotein A-II/ApoA2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4215

R&D Systems, part of Bio-Techne
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MAB4215
MAB4215-SP

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 395906

Product Specifications

Immunogen

E. coli-derived recombinant human ApoA2
Gln24-Gln100
Accession # P02652

Specificity

Detects human ApoA2 in direct ELISAs and Western blots. Does not cross‑react with recombinant human (rh) ApoA1 or rhApoE.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Human Apolipoprotein A-II/ApoA2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Apolipoprotein A-II/ApoA2
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein A-II/ApoA2

Apolipoprotein A2 (ApoA2) is a major protein component of serum HDL. It is produced by the liver and is involved in cholesteryl ester formation and cholesterol transport from tissues to the liver. Polymorphisms of ApoA2 are associated with disorders of cholesterol and fatty acid metabolism. ApoA2 can form disulfide-linked 17 kDa homodimers and heterodimers with other apolipoproteins. Mature human ApoA2 shares 96% aa sequence identity with chimpanzee ApoA2 and 48% - 66% aa sequence identity with bovine, equine, mouse, and rat ApoA2.

Alternate Names

Alp-2, APOA2, Apolipoprotein AII, Hdl-1

Entrez Gene IDs

336 (Human); 11807 (Mouse); 25649 (Rat)

Gene Symbol

APOA2

UniProt

Additional Apolipoprotein A-II/ApoA2 Products

Product Documents for Human Apolipoprotein A-II/ApoA2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Apolipoprotein A-II/ApoA2 Antibody

For research use only

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