Human Apolipoprotein H/ApoH Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5087
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Apolipoprotein H/ApoH
Gly20-Cys345
Accession # P02749
Gly20-Cys345
Accession # P02749
Specificity
Detects human Apolipoprotein H/ApoH in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Apolipoprotein H/ApoH Antibody
Detection of Human Apolipoprotein H/ApoH by Western Blot.
Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL Goat Anti-Human Apolipoprotein H/ApoH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5087) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human ApoH (5 ng) was included. A specific band for Apolipoprotein H/ ApoH was detected at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Apolipoprotein H/ApoH in Mouse Pancreas.
Apolipoprotein H/ApoH was detected in perfusion fixed frozen sections of adult mouse pancreas using Goat Anti-Human Apolipoprotein H/ApoH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5087) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to pancreatic islets. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Human Apolipoprotein H/ApoH by Simple WesternTM.
Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Apolipoprotein H/ApoH at approximately 69 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Apolipoprotein H/ApoH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5087) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Apolipoprotein H/ApoH Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of adult mouse pancreas
Sample: Perfusion fixed frozen sections of adult mouse pancreas
Simple Western
10 µg/mL
Sample: Human liver tissue
Sample: Human liver tissue
Western Blot
1 µg/mL
Sample: Human liver tissue
Sample: Human liver tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Apolipoprotein H/ApoH
ApoH (Apolipoprotein H; also beta2-Glycoprotein 1/ beta2-GPI) is a 50 kDa, secreted, variably glycosylated, monomeric member of the complement control superfamily of molecules. It is produced by hepatocytes and is bound to HDL particles. Circulating ApoH blocks the intrinsic clotting pathway, and binds to exposed phospholipids on apoptotic cells. Mature human ApoH is 326 amino acids (aa) in length (aa 20‑345). It contains four Sushi repeats (aa 21‑260) and one C-terminal kringle domain (aa 261‑345) that binds heparin and phospholipids. At least two ApoH isoforms exist, one showing a 31 aa substitution for aa 140‑345, while a second represents a bioactive plasmin cleavage product that removes the C-terminal nine amino acids. Mature human ApoH shares 76% aa identity with mouse ApoH.
Alternate Names
APOH, B2G1, B2GPI, Beta, BG
Gene Symbol
APOH
UniProt
Additional Apolipoprotein H/ApoH Products
Product Documents for Human Apolipoprotein H/ApoH Antibody
Product Specific Notices for Human Apolipoprotein H/ApoH Antibody
For research use only
Loading...
Loading...
Loading...
Loading...