Human BAI1 Alexa Fluor® 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB49691R

R&D Systems, part of Bio-Techne
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FAB49691R-100UG
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 480912

View all BAI1 Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human BAI1
Ala31-Thr879
Accession # O14514

Specificity

Detects human BAI1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human BAI1 Alexa Fluor® 647-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human BAI1 and eGFP
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: BAI1

Brain Angiogenesis Inhibitor 1 (BAI1) is a 170 kDa 7-transmembrane domain G protein-coupled receptor (GPCR) that has a large N-terminal extracellular region with an RGD motif, five thrombospondin type I repeats, and a juxtamembrane GPS (GPCR proteolytic cleavage site) (1). Within the extracellular domain (ECD) up to the GPS (amino acids 31‑879), mature human BAI1 shares 94% amino acid sequence identity with mouse and rat BAI1. BAI1 is preferentially expressed on brain neurons but also is found on astrocytes and macrophages and in the pancreas, stomach, and colon (1‑8). BAI1 can be cleaved within the GPS to release a 120 kDa fragment termed Vasculostatin which corresponds to nearly the entire N-terminal ECD (9). Generation of additional soluble fragments suggests the cleavage of BAI1 at multiple sites (9, 10). BAI1 fragments interact with Integrin alphaV beta5 or CD36 on microvascular endothelial cells to inhibit cell proliferation and migration (10, 11). Overexpression of BAI1 in glioblastoma or pancreatic adenocarcinoma cells inhibits their tumorigenicity and the development of tumor-associated neovascularization (6, 12). Fragments of the ECD, including Vasculostatin, also suppress in vivo angiogenesis and tumor growth (1, 9, 11). BAI1 is down‑regulated in glioblastoma, carcinomas of the pancreas, colon, and stomach and also in experimental ischemia (2, 4, 6‑8). Its expression is inversely correlated with tumor vascularity in colorectal and pulmonary carcinomas (8, 13). On macrophages and astrocytes, BAI1 mediates the phagocytosis of apoptotic cells through recognition of cell surface phosphatidylserine (5).

References

  1. Nishimori, H. et al. (1997) Oncogene 15:2145.
  2. Koh, J.T. et al. (2001) Brain Res. Mol. Brain Res. 87:223.
  3. Mori, K. et al. (2002) Neurosci. Res. 43:69.
  4. Kaur, B. et al. (2003) Am. J. Pathol. 162:19.
  5. Park, D. et al. (2007) Nature 450:430.
  6. Duda, D.G. et al. (2002) Br. J. Cancer 86:490.
  7. Lee, J.H. et al. (2001) Int. J. Oncol. 18:355.
  8. Fukushima, Y. et al. (1998) Int. J. Oncol. 13:967.
  9. Kaur, B. et al. (2005) Oncogene 24:3632.
  10. Koh, J.T. et al. (2004) Exp. Cell Res. 294:172.
  11. Kaur, B. et al. (2009) Cancer Res. 69:1212.
  12. Kang, X. et al. (2006) Cancer Gene Ther. 13:385.
  13. Hatanaka, H. et al. (2000) Int. J. Mol. Med. 5:181.

Long Name

Brain-specific Angiogenesis Inhibitor 1

Alternate Names

brain-specific angiogenesis inhibitor 1, FLJ41988, GDAIF

Entrez Gene IDs

575 (Human); 107831 (Mouse); 362931 (Rat)

Gene Symbol

ADGRB1

UniProt

O14514

Additional BAI1 Products

Product Documents for Human BAI1 Alexa Fluor® 647-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human BAI1 Alexa Fluor® 647-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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