Human BAI1 Antibody

Detection of Mouse BAI1 by Immunocytochemistry/Immunofluorescence

Bai1Tg reduces accumulation of apoptotic corpses in Mertk−/− mice post-torsion. (A) Sertoli cell expression of Bai1, BAI1 signaling pathway genes, and Mertk were analyzed by quantitative RT-PCR. Sertoli cells were isolated from Mertk+/+ (n = 4) and Mertk−/− (n = 2) mice and were cultured for 3 days to expand them prior to RNA isolation. Error bars are standard error of mean (SEM). (B) Representative images of isolated Sertoli cells from Mertk−/− and Mertk−/−Bai1Tg mice were stained for BAI1 to confirm surface expression of the Bai1Tg. (C) Mice (8–12 weeks-old) underwent testicular torsion surgery to induce ischemic injury. Testicular cross sections from sham and torsion testes were stained for cleaved caspase 3 (CC3) (black arrowheads). Images are of representative tubule cross sections from matched sham and torsion testes. (D) The number of CC3 positive cells per tubule cross section was determined by analyzing the entire testicular cross section. Each mouse is represented by individual data points within the bars. Mertk+/+ (n = 5) Mertk−/− (n = 8) Mertk−/−Bai1Tg (n = 9). Error bars represent SEM. Statistical analysis was performed with a Wilcoxon rank-sum test. *p < 0.05, ***p < 0.001. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-017-15191-1), licensed under a CC-BY license. Not internally tested by R&D Systems.