Human BATF APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC8054A

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 687706

View all BATF Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human BATF
Met1-Ser125
Accession # Q16520

Specificity

Detects Human BATF in ELISA and Western Blot.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human BATF APC-conjugated Antibody

Detection of BATF antibody in Raji Human Cell Line antibody by Flow Cytometry.

Detection of BATF in Raji Human Cell Line by Flow Cytometry.

Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human BATF APC-conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # IC8054A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human BATF APC-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Raji human Burkitt's lymphoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: BATF

BATF is a member of the AP-1 family of basic leucine zipper transcription factors. It associates with c-Jun proteins to form heterodimeric factors that inhibit transcription at AP-1 sites. The DNA binding of BATF is regulated by its phosphorylation at Ser43. BATF plays a key role in several aspects of immune system development. It suppresses the differentiation of NKT and iNKT cells, while it promotes the differentiation of Th17, Th2, follicular Th cells, CD8+ T cells, and CD8+ dendritic cells. It is required for class-switch recombination in B cells and T cells as well as for germinal center formation and B cell maturation. It is also required for the expression of T cell surface proteins that mediate homing of Th cells to the gut. BATF cooperates with IRF4 in binding to composite DNA elements that are responsive to both IRF4 and AP1. Human BATF1 shares 96% amino acid sequence identity with mouse and rat BATF1

Long Name

Activating Transcription Factor 1

Alternate Names

SFA2

Entrez Gene IDs

10538 (Human); 53314 (Mouse); 299206 (Rat)

Gene Symbol

BATF

UniProt

Q16520

Additional BATF Products

Product Documents for Human BATF APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human BATF APC-conjugated Antibody

For research use only

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