Human beta-Defensin 4/2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2758
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human beta-Defensin 4/2
Gly24-Pro64
Accession # O15263
Gly24-Pro64
Accession # O15263
Specificity
Detects human beta-Defensin 4/2 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human beta-Defensin 4/2 Antibody
beta-Defensin 4/2 in Human Lung.
beta-Defensin 4/2 was detected in immersion fixed paraffin-embedded sections of human lung using Human beta-Defensin 4/2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2758) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of alveolar cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
beta-Defensin 4/2 in Human Kidney Array.
beta-Defensin 4/2 was detected in immersion fixed paraffin-embedded sections of human kidney array using Human beta-Defensin 4/2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2758) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human beta-Defensin 4/2 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung and immersion fixed paraffin-embedded sections of human kidney array
Sample: Immersion fixed paraffin-embedded sections of human lung and immersion fixed paraffin-embedded sections of human kidney array
Western Blot
0.1 µg/mL
Sample: Recombinant Human beta-Defensin 4/2
Sample: Recombinant Human beta-Defensin 4/2
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-Defensin 2
Long Name
Defensin 2 beta
Alternate Names
BD-2, betaDefensin 2, DEFB2, DEFB4A, DEFB4B, HBD-2, SAP-1
Entrez Gene IDs
1673 (Human)
Gene Symbol
DEFB4A
UniProt
Additional beta-Defensin 2 Products
Product Documents for Human beta-Defensin 4/2 Antibody
Product Specific Notices for Human beta-Defensin 4/2 Antibody
For research use only
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