Human BMAL1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10560
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Met105-Glu303
Accession # O00327
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human BMAL1 Antibody
Human BMAL1 ELISA Standard Curve.
Recombinant Human BMAL1 protein was serially diluted 2-fold and captured by Mouse Anti-Human BMAL1 Monoclonal Antibody (MAB105601) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human BMAL1 Monoclonal Antibody (Catalog # MAB10560) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).Applications for Human BMAL1 Antibody
ELISA
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human BMAL1 Monoclonal Antibody (Catalog # MAB105601).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Formulation, Preparation, and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMAL1
BMAL1 (Brain and Muscle ARNT-like 1; ARNTL) is an essential core component in circadian clock machinery which is regulatory mechanism for circadian rhythms. Circadian clock genes include three period proteins (PER1, PER2 and PER3), two cryptochromes (CRY1 and CRY2), CLOCK, NPAS2 and BMAL proteins, wherein BMAL1 has a potential to heterodimerize with CLOCK or NPAS2 genes; and this neocomplex drives transcription from E-box elements (5'-CACGTG-3') found in circadian-responsive genes's promoters. PER/CRY proteins negatively regulate CLOCK/BMAL1 dimer-mediated transcription, thereby forming the feedback loop that regulates the timing of clock gene transcription. BMAL1 also associates with GNB2L1/RACK1 and PRKCA in a nuclear complex, whertein GNB2L1 and PRKCA are recruited to the complex in a circadian manner. BMAL1 undergoes acetylation (Lys-538), phosphorylation and sumoylation (Lys-259) upon dimerization with CLOCK and acetylation facilitates CRY1-mediated repression. CLOCK-BMAL1 double mutations within PAS domains leads to syngernistic desensitization to high levels of CRY on repression of CLOCK-BMAL1 transcriptional activity of PER1 and, disrupt circadian rhythmicity. Clock genes functions primarily as tumor suppressors and their abbarant expression is observed in malignant pleural mesothelioma, colorectal and breast cancer.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional BMAL1 Products
Product Documents for Human BMAL1 Antibody
Product Specific Notices for Human BMAL1 Antibody
For research use only