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Human BMP-8 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF1073

R&D Systems, part of Bio-Techne
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AF1073
AF1073-SP

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant human BMP‑8a
Ala264-His402
Accession # AAP74559

Specificity

Detects human BMP-8 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) BMP-2, rhBMP-3, rhBMP-4, rhBMP-5, rhBMP-6 and rhBMP-7 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human BMP-8 Antibody

BMP-8 antibody in Human Osteo-sarcoma by Immunohistochemistry (IHC-P).

BMP‑8 in Human Osteo-sarcoma.

BMP-8 was detected in immersion fixed paraffin-embedded sections of human osteosarcoma using 15 µg/mL Goat Anti-Human BMP-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1073) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human BMP-8 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human osteosarcoma

Western Blot

0.1 µg/mL
Sample: Recombinant Human BMP-8

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-8

BMP-8, also known as osteogenic protein 2 (OP-2), was first isolated from a hippocampal library in a screen to identify relatives of BMP-7 (OP-1) (1). BMPs are a family of structurally and functionally related proteins and represent a subfamily of the transforming growth factor beta (TGF-beta) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. They have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs (2). BMPs play roles in regulating growth, differentiation, chemotaxis, and apoptosis of various cell types, including mesenchymal, epithelial, hematopoetic, and neuronal cells.

There exist two highly related and closely linked genes, designated BMP-8a and -8b in mice and humans. For humans, the protein products of these two genes share 98% amino acid (aa) sequence identity in their pro- and mature regions. However in the mouse, the two proteins share 89% and 76% aa sequence homology in their pro- and mature regions, respectively (3). Mature human BMP-8a shares 91% and 70% aa sequence identity with mouse BMP-8a and -8b, respectively. Human BMP‑8a is synthesized as a large precursor protein that is cleaved at a dibasic cleavage site (RTPR) between aa residues 263 and 264 to release a 139 aa carboxy-terminal domain. Expression patterns of the BMP-8 genes indicate that they regulate aspects of cell proliferation and/or differentiation during spermatogenesis and formation of the placenta (3). BMP-8 is also highly expressed in osteosarcomas (4).

References

  1. Ozkaynak, E. et al. (1992) J. Biol. Chem. 267:25220.
  2. Canalis, E. et al. (2003) Endocrine Rev. 24:218.
  3. Zhao, G-Q. et al. (1996) Mech. Dev. 57:159.
  4. Sulzbacher, I. et al. (2002) J. Clin. Pathol. 55:381.

Long Name

Bone Morphogenetic Protein 8

Alternate Names

BMP8, BMP8A, OP-2

Entrez Gene IDs

353500 (Human); 12163 (Mouse)

Gene Symbol

BMP8A

UniProt

Additional BMP-8 Products

Product Documents for Human BMP-8 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human BMP-8 Antibody

For research use only

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