Human BMPR-II Antibody
R&D Systems, part of Bio-Techne | Catalog # AF811
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Rabbit
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunohistochemistry
Cited:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BMPR-II
Ala26-Ile151
Accession # Q13873
Ala26-Ile151
Accession # Q13873
Specificity
Detects human BMPR-II in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human BMPR-II Antibody
BMPR-II in Human Prostate.
BMPR-II was detected in formalin fixed paraffin-embedded sections of human prostate using Goat Anti-Human BMPR-II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF811) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of BMPR-II in PC-3 Human Cell Line by Flow Cytometry.
PC-3 human prostate cancer cell line was stained with Goat Anti-Human BMPR-II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF811, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).Applications for Human BMPR-II Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: PC‑3 human prostate cancer cell line
Sample: PC‑3 human prostate cancer cell line
Immunohistochemistry
5-15 µg/mL
Sample: Immersion-fixed frozen sections of rat embryo and immersion fixed paraffin-embedded sections of human prostate
Sample: Immersion-fixed frozen sections of rat embryo and immersion fixed paraffin-embedded sections of human prostate
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMPR-II
Like other members of the TGF-beta superfamily, cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the binding to heteromeric complex of type I and type II serine-threonine kinase receptors. Both receptor types are required for the signal transduction. BMP receptor II (BMPR-II) is one of the five mammalian type II receptors (including TGF-beta R-II, ActR-II, ActR-IIB, BMPR-II, and MISR-II) for the TGF-beta superfamily ligands. The type II receptors for TGF-beta and activin bind ligands with high affinity by itself. In contrast, BMPR-II binds BMP-2, BMP-4, and BMP-7 weakly in the absence of type I receptor, and the binding can be facilitated by the presence of the type I receptor. BMPR-II mRNA is widely expressed in fetal and adult tissues. Human and mouse BMPR-II are highly conserved sharing 97% sequence identity (1-3).
References
- Rosenzweig, B.L. et al. (1995) Proc. Natl. Acad. Sci. USA 92:7632.
- Beppu, H. et al. (1997) Biochem. Biophys. Res. Commun. 235:499.
- Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.
Long Name
Bone Morphogenetic Protein Receptor II
Alternate Names
BMPR2, BMPRII
Gene Symbol
BMPR2
UniProt
Additional BMPR-II Products
Product Documents for Human BMPR-II Antibody
Product Specific Notices for Human BMPR-II Antibody
For research use only
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