Human BRIP1/FANCJ Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6496
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 652747
Product Specifications
Immunogen
E. coli-derived recombinant human BRIP1/FANCJ
Ser2-Arg160
Accession # Q9BX63
Ser2-Arg160
Accession # Q9BX63
Specificity
Detects human BRIP1/FANCJ in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant
human (rh) FANCA, D2, E, F, G, I, L, M, or recombinant mouse BRIP1/FANCJ is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human BRIP1/FANCJ Antibody
Detection of Human BRIP1/FANCJ by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF-7 human breast cancer cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human BRIP1/FANCJ Monoclonal Antibody (Catalog # MAB6496) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for BRIP1/FANCJ at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
BRIP1/FANCJ in Human Breast Cancer Tissue.
BRIP1/FANCJ was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human BRIP1/FANCJ Monoclonal Antibody (Catalog # MAB6496) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hemotoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human BRIP1/FANCJ Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, MCF‑7 human breast cancer cell line, and HeLa human cervical epithelial carcinoma cell line
Sample: Jurkat human acute T cell leukemia cell line, MCF‑7 human breast cancer cell line, and HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BRIP1/FANCJ
Long Name
BRCA1 Interacting Protein C-terminal Helicase 1
Alternate Names
BACH1, FACJ, FANCJ
Entrez Gene IDs
83990 (Human)
Gene Symbol
BRIP1
UniProt
Additional BRIP1/FANCJ Products
Product Documents for Human BRIP1/FANCJ Antibody
Product Specific Notices for Human BRIP1/FANCJ Antibody
For research use only
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