Human BRIX Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4024

R&D Systems, part of Bio-Techne
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AF4024
AF4024-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all BRIX Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human BRIX
Ser131-Lys353
Accession # Q8TDN6

Specificity

Detects human BRIX in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human BRIX Antibody

Detection of Human BRIX antibody by Western Blot.

Detection of Human BRIX by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human BRIX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4024) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for BRIX at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human BRIX Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BRIX

BRIX (Biogenesis of ribosomes in Xenopus; also BRIX domain-containing protein 2) is a 41 kDa (predicted) member of the BXDC2 family of proteins. It is found in nucleoli, and appears to participate in the formation of a complex associated with pre-rRNA and enzymes that generate 60S ribosomal subunits. Human BRIX is 353 amino acids (aa) in length. It is characterized by the presence of a BRIX domain that lies between aa 60‑249. There is one splice variant that shows a 17 aa substitution for aa 11‑27. Over aa 131‑353, human BRIX shares 93% aa identity with mouse BRIX.

Long Name

Brix Domain Containing 2

Alternate Names

BRIX1, BXDC2

Entrez Gene IDs

55299 (Human); 67832 (Mouse); 294799 (Rat)

Gene Symbol

BRIX1

UniProt

Q8TDN6

Additional BRIX Products

Product Documents for Human BRIX Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human BRIX Antibody

For research use only

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