Human c-Rel Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4606
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 321422
Product Specifications
Immunogen
E. coli-derived recombinant human c-Rel
Met1-Val619
Accession # NP_002899
Met1-Val619
Accession # NP_002899
Specificity
Detects human c-Rel in Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human c-Rel Antibody
Detection of Human c-Rel by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rat Anti-Human c-Rel Monoclonal Antibody (Catalog # MAB4606) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for c-Rel at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
c-Rel in Human PBMCs.
c-Rel was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 15 µg/mL Rat Anti-Human c-Rel Monoclonal Antibody (Catalog # MAB4606) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with nuclear stain (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human c-Rel Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)
Western Blot
0.1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and Raji human Burkitt's lymphoma cell line
Sample: K562 human chronic myelogenous leukemia cell line and Raji human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: c-Rel
c-Rel/Rel, the cellular counterpart of the v-Rel oncogene of the avian reticuloendotheliosis retrovirus, is a 85 kDa class II member of the Rel/NK-kappa B family of transcription factors. All Rel family members contain a RHD (Rel homology domain) that is involved in dimerization, DNA and I kappaB binding, and nuclear localization. Class II members contain an additional C-terminal transcriptional activation segment. Rel both homodimerizes and heterodimerizes with multiple family members. Following dimerization, Rel complexes initiate transcription by acting on decameric DNA motifs termed kappaB binding sites. c-Rel activity is regulated by phosphorylation and ubiquitination. The important role of c-Rel in B-cell development, growth, and survival is well documented. c-Rel is also involved in responses to auto-antigens, allo-antigens, allergens and pathogens and may contribute to the development of certain human lymphoid cell cancers.
Long Name
Cellular Repressor of E1A-stimulated Genes
Alternate Names
cRel, REL
Gene Symbol
REL
UniProt
Additional c-Rel Products
Product Documents for Human c-Rel Antibody
Product Specific Notices for Human c-Rel Antibody
For research use only
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