Human C1D Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4816
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human C1D
Ala2-Ser141
Accession # Q13901
Ala2-Ser141
Accession # Q13901
Specificity
Detects human C1D in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human C1D Antibody
Detection of Human C1D by Western Blot.
Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human C1D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4816) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for C1D at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human C1D Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: JEG-3 human epithelial choriocarcinoma cell line
Sample: JEG-3 human epithelial choriocarcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: C1D
C1D (also SUN-CoR) is a 16 kDa member of the C1D family of DNA-binding proteins. It is ubiquitously expressed, activates DNA‑dependent protein kinase, and forms part of a large complex that processes rRNA. C1D exists as both a monomer and noncovalent homodimer, and is known to be phosphorylated. Human C1D is 141 amino acids (aa) in length. It contains two protein interaction sites at aa 50‑100 and 101‑141. There is one potential alternate site at Met54, and a number of single aa polymorphisms. Full-length C1D is 90% and 87% aa identical to mouse and canine C1D, respectively.
Long Name
C1D Nuclear DNA-binding Protein
Alternate Names
SUNCOR
Gene Symbol
C1D
UniProt
Additional C1D Products
Product Documents for Human C1D Antibody
Product Specific Notices for Human C1D Antibody
For research use only
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