Human Calbindin D Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3320
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Simple Western, Western Blot
Cited:
ELISA Development (Capture)
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 401025
Product Specifications
Immunogen
E. coli-derived recombinant human Calbindin D
Met1-Asn261
Accession # P05937
Met1-Asn261
Accession # P05937
Specificity
Detects human Calbindin D in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human S100P.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Calbindin D Antibody
Detection of Human Calbindin D by Western Blot.
Western blot shows lysates of human kidney tissue and human brain (cerebellum) tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Calbindin D Monoclonal Antibody (Catalog # MAB3320) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Calbindin D at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Calbindin D in Rat Brainstem.
Calbindin D was detected in perfusion fixed paraffin-embedded sections of rat brainstem (medulla) using 25 µg/mL Mouse Anti-Human Calbindin D Monoclonal Antibody (Catalog # MAB3320) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Calbindin D by Simple WesternTM.
Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Calbindin D at approximately 32 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Calbindin D Monoclonal Antibody (Catalog # MAB3320) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Calbindin D Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of rat brain (hypothalamus) and brainstem (medulla), and immersion fixed paraffin-embedded sections of human brain (cerebellum)
Sample: Perfusion fixed frozen sections of rat brain (hypothalamus) and brainstem (medulla), and immersion fixed paraffin-embedded sections of human brain (cerebellum)
Simple Western
10 µg/mL
Sample: Human brain (cerebellum) tissue
Sample: Human brain (cerebellum) tissue
Western Blot
0.25 µg/mL
Sample: Human kidney tissue and human brain (cerebellum) tissue
Sample: Human kidney tissue and human brain (cerebellum) tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Calbindin D
Long Name
Vitamin D-dependent Calcium-Binding Protein
Alternate Names
CABP, CABP1, CABP28K, CABP9K, Calbindin 3, Calbindin-D, Calbindin-D9K, S100G
Gene Symbol
S100G
UniProt
Additional Calbindin D Products
Product Documents for Human Calbindin D Antibody
Product Specific Notices for Human Calbindin D Antibody
For research use only
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