Human Carbohydrate Sulfotransferase 5/CHST5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5446
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Carbohydrate Sulfotransferase 5/CHST5
Ser28-Arg390
Accession # Q9GZS9
Ser28-Arg390
Accession # Q9GZS9
Specificity
Detects human Carbohydrate Sulfotransferase 5/CHST5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) CHST1, rhCHST2, rmCHST5, and rmCHST7 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Carbohydrate Sulfotransferase 5/CHST5 Antibody
Detection of Human Carbohydrate Sulfotransferase 5/CHST5 by Western blot.
Western blot shows lysates of HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carbohydrate Sulfotransferase 5/CHST5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5446) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Carbohydrate Sulfotransferase 5/CHST5 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human Carbohydrate Sulfotransferase 5/CHST5 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carbohydrate Sulfotransferase 5/CHST5, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Carbohydrate Sulfotransferase 5/CHST5, see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: HT-29 human colon adenocarcinoma cell line
Sample: HT-29 human colon adenocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbohydrate Sulfotransferase 5/CHST5
Alternate Names
CHST5, GlcNAc6ST3, GST4-alpha, hIGn6ST, I-GlcNAc6ST
Gene Symbol
CHST5
UniProt
Additional Carbohydrate Sulfotransferase 5/CHST5 Products
Product Documents for Human Carbohydrate Sulfotransferase 5/CHST5 Antibody
Product Specific Notices for Human Carbohydrate Sulfotransferase 5/CHST5 Antibody
For research use only
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