Human Carbonic Anhydrase VI Alexa Fluor® 594-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC29391T
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 401809
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase VI
Gln18-Asn308
Accession # EAW71606
Gln18-Asn308
Accession # EAW71606
Specificity
Detects human Carbonic Anhydrase VI in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Carbonic Anhydrase 1, 2, 3, 4, 5A, 5B, 7, 8, or 9 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human Carbonic Anhydrase VI Alexa Fluor® 594-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Sample: OVCAR‑3 human ovarian carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: OVCAR‑3 human ovarian carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Carbonic Anhydrase VI
Carbonic Anhydrase catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. Carbonic Anhydrase VI, also known as gustin and salivary Carbonic Anhydrase, is a zinc‑metalloprotein that constitutes about 3% of human parotid saliva protein (2, 3). It was decreased in patients with loss of taste and pathological changes in taste buds (4). It is also an elementary component of milk. It plays an important role in normal growth and development of the infant alimentary tract (5).
References
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Murakami, H. and Sly, W. S. (1987) J. Biol. Chem. 262:1382.
- Thatcher, B. J. et al. (1998) Biochem. Biophys. Res. Commun. 250:635.
- Hankin, R. I. et al. (1999) Am. J. Med. Sci. 318:380.
- Karhumaa, P. et al. (2001) Proc. Natl. Acad. Sci. USA. 98:11604.
Alternate Names
CA6, Gustin
Gene Symbol
CA6
UniProt
Additional Carbonic Anhydrase VI Products
Product Documents for Human Carbonic Anhydrase VI Alexa Fluor® 594-conjugated Antibody
Product Specific Notices for Human Carbonic Anhydrase VI Alexa Fluor® 594-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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