Human Carbonic Anhydrase VI Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2939
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 401819
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase VI
Gln18-Asn308
Accession # EAW71606
Gln18-Asn308
Accession # EAW71606
Specificity
Detects human Carbonic Anhydrase VI in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Carbonic Anhydrase 2, 3, 4, 5A, 5B, 7, 8, 9, recombinant mouse Carbonic Anhydrase 12, and 14 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Carbonic Anhydrase VI Antibody
Detection of Human Carbonic Anhydrase VI by Western Blot.
Western blot shows human saliva. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Carbonic Anhydrase VI Monoclonal Antibody (Catalog # MAB2939) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Carbonic Anhydrase VI at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Carbonic Anhydrase VI by Simple WesternTM.
Simple Western lane view shows human saliva, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase VI at approximately 56 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Carbonic Anhydrase VI Monoclonal Antibody (Catalog # MAB2939) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.Applications for Human Carbonic Anhydrase VI Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase VI (Catalog # 2939-CA), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase VI (Catalog # 2939-CA), see our available Western blot detection antibodies
Simple Western
10 µg/mL
Sample: Human saliva
Sample: Human saliva
Western Blot
1 µg/mL
Sample: Human saliva
Sample: Human saliva
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase VI
Carbonic Anhydrase catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Carbonic Anhydrase VI, also known as gustin and salivary Carbonic Anhydrase, is a zinc-metalloprotein that constitutes about 3% of human parotid saliva protein (2, 3). It was decreased in patients with loss of taste and pathological changes in taste buds (4). It is also an elementary component of milk. It plays an important role in normal growth and development of the infant alimentary tract (5). Mature human CA6 shares 66% and 50% amino acid sequence identity with bovine and mouse CA6, respectively.
References
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Murakami, H. and Sly, W. S. (1987) J. Biol. Chem. 262:1382.
- Thatcher, B. J. et al. (1998) Biochem. Biophys. Res. Commun. 250:635.
- Hankin, R. I. et al. (1999) Am. J. Med. Sci. 318:380.
- Karhumaa, P. et al. (2001) Proc. Natl. Acad. Sci. USA. 98:11604.
Alternate Names
CA6, Gustin
Gene Symbol
CA6
UniProt
Additional Carbonic Anhydrase VI Products
Product Documents for Human Carbonic Anhydrase VI Antibody
Product Specific Notices for Human Carbonic Anhydrase VI Antibody
For research use only
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