Human Carbonic Anhydrase XIV/CA14 Antibody

Name: Human Carbonic Anhydrase XIV/CA14 Antibody
Brand: R&D Systems
SKU: MAB2195

R&D Systems, part of Bio-Techne | Catalog # MAB2195

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 312908

View all Carbonic Anhydrase XIV/CA14 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase XIV/CA14
Gly19-Met290
Accession # Q9ULX7

Specificity

Detects human Carbonic Anhydrase XIV/CA14 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross‑reactivity with recombinant mouse CA14, 10% cross‑reactivity with recombinant human (rh) CA5A, and no cross-reactivity with rhCA1, 2, 3, 4, 6, 7, 8, 9, 10, 12, or 13 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Applications for Human Carbonic Anhydrase XIV/CA14 Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase XIV (Catalog # 2195-CA), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Carbonic Anhydrase XIV (Catalog # 2195-CA)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase XIV/CA14

Carbonic Anhydrase 14 (CA14) has a C-terminal transmembrane domain (aa 291 - 311) and a short cytoplasmic tail (aa 312 - 337). As a functioning enzyme of the CA family, it catalyzes the reversible hydration of CO2. CA14 is highly expressed in all parts of the central nervous system with lower expression in adult liver, heart, small intestine, colon, kidney, urinary bladder and skeletal muscle. The rhCA14 corresponds to the 272 aa ectodomain that has a predicted molecular mass of approximately 32 kDa and an apparent molecular mass of approximately 43 kDa in SDS-PAGE. The amino acid sequence of the human ectodomain shares 98%, 86%, 84%, and 83% identity with that of chimpanzee, bovine/dog, rat and mouse, respectively.

Alternate Names

CA14

Entrez Gene IDs

23632 (Human); 23831 (Mouse)

Gene Symbol