Human Carboxypeptidase B1/CPB1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2897
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 438806
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase B1/CPB1
His16-Tyr417
Accession # P15086
His16-Tyr417
Accession # P15086
Specificity
Detects human Carboxypeptidase B1/CPB1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CPA1, rhCPA2, rhCPE, recombinant mouse (rm) CPA4, and rmCPB1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Human Carboxypeptidase B1/CPB1 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carboxypeptidase B1/CPB1 (Catalog # 2897-ZN), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Carboxypeptidase B1/CPB1 (Catalog # 2897-ZN), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Carboxypeptidase B1/CPB1 (Catalog # 2897-ZN)
Sample: Recombinant Human Carboxypeptidase B1/CPB1 (Catalog # 2897-ZN)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carboxypeptidase B1/CPB1
Carboxypeptidase B1, encoded by the CPB1 gene, specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg (1). Expressed mainly in pancreas, CPB1 is a useful serum marker for acute pancreatitis (2). The deduced amino acid (aa) sequence of human CPB1 consists of a signal peptide (aa 1-15), a pro region (aa 16-110), and a mature chain (aa 111-417). Human CPB1 shares 98%, 84%, 76%, 75%, and 84% aa sequence identity with chimpanzee, dog, rat, mouse and chicken CPB1, respectively.
References
- Aviles, F.X. and J. Vendrell (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) p. 831, Academic Press, San Diego.
- Yamamoto, K.K. et al. (1992) J. Biol. Chem. 267:2575.
Alternate Names
CPB1, PASP, PCPB
Gene Symbol
CPB1
UniProt
Additional Carboxypeptidase B1/CPB1 Products
Product Documents for Human Carboxypeptidase B1/CPB1 Antibody
Product Specific Notices for Human Carboxypeptidase B1/CPB1 Antibody
For research use only
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