Human Carboxypeptidase E/CPE Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3587
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 420709
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase E/CPE
Arg42-Ser453
Accession # P16870
Arg42-Ser453
Accession # P16870
Specificity
Detects human Carboxypeptidase E/CPE in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CPA1, rhCPA2, rhCPB1, or recombinant mouse CPA4 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Human Carboxypeptidase E/CPE Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carboxypeptidase E/CPE (Catalog # 3587-ZN), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Carboxypeptidase E/CPE (Catalog # 3587-ZN), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Carboxypeptidase E/CPE (Catalog # 3587-ZN)
Sample: Recombinant Human Carboxypeptidase E/CPE (Catalog # 3587-ZN)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carboxypeptidase E/CPE
Carboxypeptidase E (CPE) is a peripheral membrane protein that cleaves C-terminal amino acid residues and is involved in the processing of numerous peptide hormones and neurotransmitters. CPE knockout mice live but become obese due to impaired glucose clearance and insulin resistance. The CPE precursor includes a signal peptide (aa 1‑25), a propeptide (aa 26‑42) and a mature chain (aa 43‑476). The amino acid sequence of human CPE is 99.8%, 96.6%, 96.3%, and 90.2% identical to that of chimpanzee, mouse, dog and chicken.
Alternate Names
CPE, CPH-1
Gene Symbol
CPE
UniProt
Additional Carboxypeptidase E/CPE Products
Product Documents for Human Carboxypeptidase E/CPE Antibody
Product Specific Notices for Human Carboxypeptidase E/CPE Antibody
For research use only
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