Human Cathepsin A/Lysosomal Carboxypeptidase A Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1049
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 179803
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin A/Lysosomal Carboxypeptidase A
Ala29-Tyr480 (predicted)
Accession # P10619
Ala29-Tyr480 (predicted)
Accession # P10619
Specificity
Detects human Cathepsin A/Lysosomal Carboxypeptidase A in direct ELISAs and Western blots. In Western blots, detects the single chain (55 kDa) and heavy chain (32 kDa) forms of recombinant human (rh) Cathepsin A. In Western blots, less than 5% cross-reactivity with rhCathepsin B, C, D, E, L, O, S, X and Z is observed and no cross-reactivity with the light chain (20 kDa) of rhCathepsin A is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Human Cathepsin A/Lysosomal Carboxypeptidase A Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Cathepsin A/Lysosomal Carboxypeptidase A (Catalog # 1049-SE), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Cathepsin A/Lysosomal Carboxypeptidase A (Catalog # 1049-SE), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Cathepsin A/Lysosomal Carboxypeptidase A (Catalog # 1049-SE)
Sample: Recombinant Human Cathepsin A/Lysosomal Carboxypeptidase A (Catalog # 1049-SE)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin A/Lysosomal Carboxypeptidase A
Cathepsin A/lyososomal carboxypeptidase A is a member of the serine carboxypeptidase family (1). Cathepsin A is a multifunctional enzyme that expresses deaminidase and esterase activities at neutral pH and carboxypeptidase activity at acidic pH. Also known as protective protein, its association with beta-galactosidase ( beta-gal) and neuraminidase is essential for beta-gal stability and neuraminidase activation in the lysosomes. Inherited deficiency of Cathepsin A causes the lysosomal storage disorder galactosialidosis, characterized by a combined secondary deficiency of beta-gal and neuraminidase. Cathepsin A is capable of hydrolyzing a variety of bioactive peptide hormones including tachykinins, indicating that extralysosomal Cathepsin A plays a role in regulation of functions of these molecules (2). Cathepsin A is synthesized as a single-chain precursor and processed into heavy (32 kDa) and light (20 kDa) chains, which are linked by disulfide bonds.
References
- Pshezhetsky, A.V. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) p. 1923, Academic Press, San Diego.
- Hiraiwa, M. (1999) Cell. Mol. Life. Sci. 56:894.
Alternate Names
CTSA, Lysosomal Carboxypeptidase A
Gene Symbol
CTSA
UniProt
Additional Cathepsin A/Lysosomal Carboxypeptidase A Products
- All Products for Cathepsin A/Lysosomal Carboxypeptidase A
- Cathepsin A/Lysosomal Carboxypeptidase A cDNA Clones
- Cathepsin A/Lysosomal Carboxypeptidase A ELISA Kits
- Cathepsin A/Lysosomal Carboxypeptidase A Lysates
- Cathepsin A/Lysosomal Carboxypeptidase A Primary Antibodies
- Cathepsin A/Lysosomal Carboxypeptidase A Proteins and Enzymes
Product Documents for Human Cathepsin A/Lysosomal Carboxypeptidase A Antibody
Product Specific Notices for Human Cathepsin A/Lysosomal Carboxypeptidase A Antibody
For research use only
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