Human Cathepsin E Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1294
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin E
Ile54-Pro396
Accession # NP_001901
Ile54-Pro396
Accession # NP_001901
Specificity
Detects human pro and active Cathepsin E in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross‑reactivity with recombinant mouse Cathepsin E is observed and less than 2% cross‑reactivity with recombinant human (rh) Cathepsin D and rhBACE is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Cathepsin E Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon
Sample: Immersion fixed paraffin-embedded sections of human colon
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Cathepsin E (Catalog # 1294-AS), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Cathepsin E (Catalog # 1294-AS), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human Cathepsin E (Catalog # 1294-AS)
Sample: Recombinant Human Cathepsin E (Catalog # 1294-AS)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin E
Cathepsin E is an intracellular aspartic protease of the pepsin family (1). Unlike Cathepsin D, another member of the same family and a lysosomal protease with relatively ubiquitous distribution, Cathepsin E is not a lysosomal enzyme and has a limited cell and tissue distribution. However, both Cathepsin D and E play an important role in the degradation of proteins, the generation of bioactive proteins, and antigen processing (2). Both enzymes are efficient in cleaving Swedish mutant of amyloid precursor protein (APP) at the beta site but show almost no reactivity with wild-type APP (3). Human Cathepsin E is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑17), a propeptide (residues 18‑53), and a mature chain (residues 54‑396) (4).
References
- Kay, J. and P.J. Tatnell (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al. eds.), p. 33, Academic Press, San Diego.
- Tsukuba, T. et al. (2000) Mol. Cells 10:601.
- Gruninger-Leitch, F. et al. (2000) Nat. Biotechnol. 18:66.
- Azuma, T. et al. (1989) J. Biol. Chem. 264:16748.
Alternate Names
CTSE
Gene Symbol
CTSE
UniProt
Additional Cathepsin E Products
Product Documents for Human Cathepsin E Antibody
Product Specific Notices for Human Cathepsin E Antibody
For research use only
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