Human CCL11/Eotaxin Alexa Fluor® 405-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC3201V
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 43915
Product Specifications
Immunogen
E. coli-derived recombinant human CCL11/Eotaxin
Specificity
Detects human CCL11/Eotaxin in Western blots. Does not cross-react with recombinant cotton rat CCL3, 4,
5, recombinant human CCL1, 3, 4, 5, 8, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24,
25, 26, 27, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 8, 9/10/MIP-1 gamma, 11, 12, 17,
19, 20, 21, 22, 24, 25, 27, or recombinant rat CCL20.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human CCL11/Eotaxin Alexa Fluor® 405-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Sample: WS‑1 human fetal skin fibroblast cell line treated with Recombinant Human TNF‑ alpha (Catalog # 210-TA) and Recombinant Human IL‑4 (Catalog # 204-IL) fixed with paraformaldehyde and permeabilized with saponin
Sample: WS‑1 human fetal skin fibroblast cell line treated with Recombinant Human TNF‑ alpha (Catalog # 210-TA) and Recombinant Human IL‑4 (Catalog # 204-IL) fixed with paraformaldehyde and permeabilized with saponin
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL11/Eotaxin
CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human CCL11 have been isolated. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature human CCL11. At the protein sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. In addition, human CCL11 also shows high amino acid sequence identity to human MCP-1, 2 and 3. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor (1-3). CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates (4).
References
- Kitamura, M. et al. (1996) J. Biol. Chem 271:7725.
- Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
- Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.
- Choe, H. et al. (1996) Cell 85:1135.
Alternate Names
Eotaxin
Gene Symbol
CCL11
Additional CCL11/Eotaxin Products
Product Documents for Human CCL11/Eotaxin Alexa Fluor® 405-conjugated Antibody
Product Specific Notices for Human CCL11/Eotaxin Alexa Fluor® 405-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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