Human CCL17/TARC Alexa Fluor® 700-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC3641N
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 54015
Product Specifications
Immunogen
E. coli-derived recombinant human CCL17/TARC
Ala24-Ser94
Accession # Q92583.1
Ala24-Ser94
Accession # Q92583.1
Specificity
Detects human CCL17/TARC in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant
human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24,
25, 26, 27, 28, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 17, 19,
20, 21, 22, 24, 25, 27 or recombinant rat CCL20
is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human CCL17/TARC Alexa Fluor® 700-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Sample: A549 human lung carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: A549 human lung carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL17/TARC
CCL17, also called TARC, is a member of the CC chemokine family. CCL17 cDNA encodes a highly basic 94 amino acid (aa) residue precursor protein with a 23 aa residue signal peptide that is cleaved to generate the 71 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24‑29% amino acid sequence identity with CCL1, 2, 3, 4, 5, 7 and 8. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. CCL17 is constitutively expressed in thymus, and at a lower level in lung, colon, and small intestine. CCL17 is also transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant CCL17 has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL17 was identified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells.
References
- Imai, T. et al. (1997) J. Biol. Chem. 272:15036.
- Imai, T. et al. (1996) J. Biol. Chem. 271:21514.
- Nomiyama, H. et al. (1997) Genomics 40:211.
Alternate Names
ABCD-2, SCYA17, TARC
Gene Symbol
CCL17
UniProt
Additional CCL17/TARC Products
Product Documents for Human CCL17/TARC Alexa Fluor® 700-conjugated Antibody
Product Specific Notices for Human CCL17/TARC Alexa Fluor® 700-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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