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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 502021

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CD14
Asp24-Leu197
Accession # Q04900

Specificity

Detects human CD164 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CD164 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for Human CD164 Antibody

Detection of CD164 antibody in CXCL12-treated PC-3 Human Cell Line antibody by Flow Cytometry.

Detection of CD164 in CXCL12-treated PC‑3 Human Cell Line by Flow Cytometry.

CXCL12-treated PC-3 human prostate cancer cell line was stained with Mouse Anti-Human CD164 Monoclonal Antibody (Catalog # MAB5790, filled histogram) or isotype control antibody (Catalog # MAB007, open histogram), followed by APC-conjugated anti-mouse antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD164 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: PC-3 human prostate cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD164

CD164, also known as endolyn, MGC-24, and MUC-24, is an 80‑100 kDa transmembrane sialomucin protein that is expressed by epithelial cells, T and B cells, monocytes, hematopoietic progenitor cells, and activated basophils. CD164 functions as an adhesion molecule and a negative regulator of cell proliferation. A soluble isoform is generated by alternate splicing. CD164 was identified as a potential regulator of homing in a prostate cancer screening for CXCL12-responsive adhesion molecules (1). Within the ECD, human CD164 shares approximately 53% sequence identity with mouse and rat CD164.

References

  1. Havens, A.M. et al. (2006) BMC Cancer 2006, 6:195.

Alternate Names

CD164, MGC-24, MUC-24

Entrez Gene IDs

8763 (Human); 53599 (Mouse)

Gene Symbol

CD164

UniProt

Additional CD164 Products

Product Documents for Human CD164 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD164 Antibody

For research use only

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