Human CD300a/LMIR1 Alexa Fluor® 750-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB2640S

R&D Systems, part of Bio-Techne
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FAB2640S-100UG
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 232612

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Product Specifications

Immunogen

Y3 rat myeloid cell line transfected with human CD300a/LMIR1

Specificity

Detects human CD300a/LMIR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross‑reactivity with recombinant human LMIR2 (CMRF-35A) is observed and no cross-reactivity with recombinant human LMIR3, 4, 5, or 6 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Human CD300a/LMIR1 Alexa Fluor® 750-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Human whole blood

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD300a/LMIR1

CD300a, also known as LMIR1 (in rodents), CMRF-35H, IRp60, CLM-8, and MAIR-I, is a 60 kDa glycoprotein member of the immunoglobulin superfamily (1). Human CD300a consists of a 163 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 98 aa cytoplasmic domain that contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and a non-canonical ITIM (2). Alternative splicing may generate additional isoforms that either lack the Ig-like domain or contain only the cytoplasmic domain. Within the ECD, human CD300a shares 40% and 43% aa sequence identity with mouse and rat LMIR1, respectively. In human, CD300a is expressed on peripheral blood eosinophils, mast cells, neutrophils, plasmacytoid dendritic cells, and various T cell subsets (3‑7). Antibody crosslinking of CD300a induces phosphorylation of tyrosine residues in the cytoplasmic domain. This leads to the recruitment of phosphatases SHIP, SHP-1, and SHP-2 and inhibition of NK cell, eosinophil, and mast cell activation (2, 3, 5‑7). Crosslinking of CD300a to other surface proteins such as SCF R or Fc epsilon RI on mast cells, Fc gamma RIIA on neutrophils, or CCR3 on mast cells and eosinophils inhibits downstream signaling from those receptors (5, 10‑12). CD300a crosslinking also limits the in vivo activities of these cells with a subsequent reduction of allergic inflammation symptoms (4, 11, 12).

References

  1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.
  2. Cantoni, C. et al. (1999) Eur. J. Immunol. 29:3148.
  3. Munitz, A. et al. (2006) Blood 107:1996.
  4. Bachelet, I. et al. (2005) J. Immunol. 175:7989.
  5. Alvarez, Y. et al. (2008) Mol. Immunol. 45:253.
  6. Ju, X. et al. (2008) Blood 112:1184.
  7. Clark, G.J. et al. (2007) J. Leukoc. Biol. 82:1126.
  8. Kumagai, H. et al. (2003) Biochem. Biophys. Res. Commun. 307:719.
  9. Yotsumoto, K. et al. (2003) J. Exp. Med. 198:223.
  10. Bachelet, I. et al. (2008) J. Immunol. 180:6064.
  11. Bachelet, I. et al. (2006) J. Allergy Clin. Immunol. 117:1314.
  12. Munitz, A. et al. (2006) J. Allergy Clin. Immunol. 118:1082.

Alternate Names

CD300a, CLM8, CMRF-35H, IGSF12, IRC1, IRC2, IRp60, LMIR1, MAIR-I, Mcipr1, NKRL, Pigr4

Entrez Gene IDs

11314 (Human); 217303 (Mouse)

Gene Symbol

CD300A

Additional CD300a/LMIR1 Products

Product Documents for Human CD300a/LMIR1 Alexa Fluor® 750-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD300a/LMIR1 Alexa Fluor® 750-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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